package wfa type Result struct { Score int CIGAR string } type Penalty struct { M int X int O int E int } type Traceback byte const ( OpenIns Traceback = iota ExtdIns OpenDel ExtdDel Sub Ins Del End ) // bitpacked wavefront lo/hi values with 32 bits each type WavefrontLoHi uint64 func PackWavefrontLoHi(lo int, hi int) WavefrontLoHi { loBM := int64(int32(lo)) & 0x0000_0000_FFFF_FFFF hiBM := int64(int64(hi) << 32) return WavefrontLoHi(hiBM | loBM) } func UnpackWavefrontLoHi(lohi WavefrontLoHi) (int, int) { loBM := int(int32(lohi & 0x0000_0000_FFFF_FFFF)) hiBM := int(int32(lohi & 0xFFFF_FFFF_0000_0000 >> 32)) return loBM, hiBM } // bitpacked wavefront values with 1 valid bit, 3 traceback bits, and 28 bits for the diag distance // technically this restricts to alignments with less than 268 million characters but that should be sufficient for most cases type WavefrontValue uint32 // TODO: add 64 bit packed value in case more than 268 million characters are needed // PackWavefrontValue: packs a diag value and traceback into a WavefrontValue func PackWavefrontValue(value uint32, traceback Traceback) WavefrontValue { validBM := uint32(0x8000_0000) tracebackBM := uint32(traceback&0x0000_0007) << 28 valueBM := value & 0x0FFF_FFFF return WavefrontValue(validBM | tracebackBM | valueBM) } // UnpackWavefrontValue: opens a WavefrontValue into a valid bool, diag value and traceback func UnpackWavefrontValue(wfv WavefrontValue) (bool, uint32, Traceback) { validBM := wfv&0x8000_0000 != 0 tracebackBM := uint8(wfv & 0x7000_0000 >> 28) valueBM := uint32(wfv & 0x0FFF_FFFF) return validBM, valueBM, Traceback(tracebackBM) } // Wavefront: stores a single wavefront, stores wavefront's lo value and hi is naturally lo + len(data) type Wavefront struct { // since wavefronts store diag distance, they should never be negative, and traceback data can be stored as uint8 data []WavefrontValue lohi WavefrontLoHi } // NewWavefront: returns a new wavefront with size accomodating lo and hi (inclusive) func NewWavefront(lo int, hi int) *Wavefront { a := &Wavefront{} a.lohi = PackWavefrontLoHi(lo, hi) size := hi - lo newData := make([]WavefrontValue, size+1) a.data = newData return a } // TranslateIndex: utility function for getting the data index given a diagonal func (a *Wavefront) TranslateIndex(diagonal int) int { lo := int(int32(a.lohi & 0x0000_0000_FFFF_FFFF)) return diagonal - lo } // Get: returns WavefrontValue for given diagonal func (a *Wavefront) Get(diagonal int) WavefrontValue { actualIdx := a.TranslateIndex(diagonal) if 0 <= actualIdx && actualIdx < len(a.data) { // idx is in the slice return a.data[actualIdx] } else { // idx is out of the slice return 0 } } // Set: the diagonal to a WavefrontValue func (a *Wavefront) Set(diagonal int, value WavefrontValue) { actualIdx := a.TranslateIndex(diagonal) /* in theory idx is always in bounds because the wavefront is preallocated if actualIdx < 0 || actualIdx >= len(a.data) { return } */ a.data[actualIdx] = value } // WavefrontComponent: each M/I/D wavefront matrix including the wavefront data, lo and hi type WavefrontComponent struct { W *PositiveSlice[*Wavefront] // wavefront diag distance and traceback for each wavefront } // NewWavefrontComponent: returns initialized WavefrontComponent func NewWavefrontComponent() *WavefrontComponent { // new wavefront component = { // lo = [0] // hi = [0] // W = [] // } w := &WavefrontComponent{ W: &PositiveSlice[*Wavefront]{ defaultValue: &Wavefront{ data: []WavefrontValue{0}, }, }, } return w } // GetVal: get value for wavefront=score, diag=k => returns ok, value, traceback func (w *WavefrontComponent) GetVal(score int, k int) (bool, uint32, Traceback) { return UnpackWavefrontValue(w.W.Get(score).Get(k)) } // SetVal: set value, traceback for wavefront=score, diag=k func (w *WavefrontComponent) SetVal(score int, k int, val uint32, tb Traceback) { w.W.Get(score).Set(k, PackWavefrontValue(val, tb)) } // GetLoHi: get lo and hi for wavefront=score func (w *WavefrontComponent) GetLoHi(score int) (bool, int, int) { lo, hi := UnpackWavefrontLoHi(w.W.Get(score).lohi) return w.W.Valid(score), lo, hi } // SetLoHi: set lo and hi for wavefront=score func (w *WavefrontComponent) SetLoHi(score int, lo int, hi int) { b := NewWavefront(lo, hi) w.W.Set(score, b) }